Hi,
    We are not using any epitope retrieval for this antibody, also the antigen
is adversely affected when in the presence of heat. Our staining procedure
uses Envision + (mouse) for 30 minuites and a 30 minute primary incubation
period (We also use the Signet antibody, I'm sure you'll find it to be a good
antibody once you get it up and running). Formerly we used LSAB+ in the same
way with the same results. As for controls we screen each patient we test and
cut a few extra on positive cases to use as controls.
Amos Brooks

"Baldridge, Lee Ann" wrote:

> Good Morning,
> Does anyone have a reliable method for Mel-5?  We are using Signet's
> conc.MEL-5(Ta99).  Have tried multiple ways to get this one to work but no
> luck.  Also, what controls are you all using?  Thanks for the help.
> Lee Ann Baldridge
> IU Hospital
> Indpls.,IN
> 317-274-5790
> 317-278-4820 fax