OK--here was my reply to the Machiavello stain--

	Pam Dilbeck
	Immunohistochemistry Section
	Washington Animal Disease Diagnostic Laboratory

---------- Forwarded message ----------
Date: Fri, 21 Apr 2000 08:47:26 -0700 (PDT)
From: Pam M. Dilbeck <pmd@vetmed.wsu.edu>
To: Renee Escalona <renee@tca.net>
Cc: Histonet <histonet@pathology.swmed.edu>
Subject: Re: Machiavello stain for rickettsia

Renee--I don't know what you are staining for--but in my experience with
Coxiella, I would choose the Pinkerton's method for rickettsias (overnight
method) over the standard Machiavello stain.  Reference: Sheehan's second
edition of Theory and practice of Histotechnology pg 242.
Pam

On Thu, 20 Apr 2000, Renee Escalona wrote:

> Hello fellow histoneters!
> 
> Does anyone out there have any experience with the Machiavello stain?  I
> found a protocol on it for smears but what we have is a paraffin block.
> Will it still work??  I have never performed this stain before so any and
> all info would be appreciated.
> 
> Thanks in advance,
> 
> Renee Escalona, BS, HTL(ASCP)
> Veterinary Laboratory Technologist
> TX Vet. Med. Diag. Lab.
> College Station, TX
> 
> 
> 

	Pam Dilbeck
	Immunohistochemistry Section
	Washington Animal Disease Diagnostic Laboratory