Re: Hale's Colloidal Iron

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From:Lee & Peggy Wenk <>
To:"Shotwell, Fernando R CIV" <>
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In case you want to look it up yourself, it is sometimes

Muller Colloidal Iron
Mowry Colloidal Iron
Muller-Mowry Colloidal Iron
Hale Colloidal Iron
or "any of the above names" dialysed iron 

Basically, you are inserting iron into mucin, then
doing the Prussian Blue iron stain to demonstrate the
iron you just put into the mucin. Usually,
nuclear fast red is the counterstain, but 
others can be used.

Basically stains the same thing as Alcian blue.
More sensitive than the alcian blue, so will 
demonstrate smaller, weaker deposits. Also seems
to be a more intense, slightly deeper blue than 
alcian blue. Takes a longer time than alcian blue, 
and some of the connective tissue is stained a 
little more than with alcian blue.

If  your lab does the iron stain (Prussian blue), 
then the only extra reagent you need is ferric 

Sorry I don't know of any where in S. CA that
you can send it out to, but I'll send you our
procedure, if you would like. Let me know.

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073

"Shotwell, Fernando R CIV" wrote:
> I work at a hospital in Southern California.  One of the pathologist at my
> lab wants a Hale's Colloidal Iron stain done on a renal specimen.  Can
> anyone help me find a reference laboratory where this procedure is done?
> And can someone point me to a website or a reference where I can learn about
> the procedure?  Thanks.
> Fernando Shotwell
> Naval Medical Center San Diego

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