Re: Embedding cells

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From:"Jeff Crews"<>
To:<>, <>
Content-Type:text/plain; charset=US-ASCII

        To hold the pellet together, you can put the pellet in Histogel (still 
sold by Richard-Allen?) and run it through the dehydration, etc as one blob of 

Jeffrey Crews, HTL (ASCP)
Organogenesis, Inc. 

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Subject: Embedding cells 
Author:  Emma Carter <> at internet
Date:    04/26/2000 4:40 PM

I am sure i read a message about this recently, but i cant recall, and i 
dont have anything in my mail folder...
someone has asked me about paraffin embedding some cells. I came up with a 
protocol off the top of my head, roughly:
Put cells in eppendorf tube, spin down and remove culture buffer. 
Resuspend with formalin and leave for 10 mins. Spin down and remove 
Resuspend/spin down through serial alcohols, then xylene.
after final resuspension with xylene, remove and cover with warm wax. 
Allow wax pellet to set. Pop out of tube and embed in cassette.
How does this appear to other people? Should i go through the dehydration 
Any help would be very appreciated...... (oh yeah, this is all to be done by 
my own fair hand.......automation!? Pah!)
Thanks in advance,
emma carter

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