Re: Embedding

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From:Amos Brooks <>
To:Jim Ball <>
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    That is one of my pet peeves too! If a specimen is improperly oriented to
start with the sections will suffer. "Theory and Practice of Histotechnology" in
the very first chapter, ironically, deals with this nicely. It helps if some
techs actually read that far.
Amos Brooks

Jim Ball wrote:

> Is there any literature on the market that shows how different tissues
> should be orientated in a block for proper cutting. I have been a tech for
> 25 years and have always taken great pride in having tissue orientated and
> embedded properly. The hospital I am currently working for the techs seem to
> only be interested in packing the molds with the as much tissue as possible,
> not to mention the chaotic arrangement of the tissue. I had only experienced
> this phenomenon before with pathologist trying to over fill cassettes. I
> really believe some one should put out a chart showing a properly cast skin
> sample, a gall bladder, a colon specimen, etc. I'am sure you have all seen
> the charts they use at repair garage when they bring out one of you spark
> plugs and compare it with a plug on a chart and tell you your motor needs
> rebuilt.
>      I can stand there all day and argue with them, but I would first like
> to have some visual as well as written evidence to back my arguments. I seem
> to remember the AFIP manual having some drawings of how tissue should be
> arranged, but if anyone has any other sources they wold like to share I
> would be in your debt. I won,t go in to the practice of putting the blocks
> on ice trays and leaving them in the freezer for any where from 15 to 30
> minutes before retrieving for sectioning. Should any one wish to do a chart
> I can furnish a ton of pictures on how not to embedd tissue.
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