RE: trans bet slides

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From:Simon Smith <ssmith@skeletech.com>
To:"Histonet (E-mail)" <histonet@pathology.swmed.edu>
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Instead of futzing with mounting media, can't you use a thick coating of
celloidin on your section, then peel that off (with tissue adhering), put
your tissue on your new slide then dissolve out the celloidin?  I seem to
remember this as a method for transferring sections from broken slides to
intact ones, rather than gluing the pieces together with extra
slides/coverslips etc.

Simon

-----Original Message-----
From: Daniel & Linda Botsford [mailto:dl.botsford@sympatico.ca]
Sent: Thursday, April 20, 2000 8:21 PM
To: P. Emry
Cc: HistoNet Server
Subject: Re: trans bet slides


Hi Trisha,
The following quote is from NSH 1999 Symposium Workshop #23 Technical
Aspects
of Diagnostic Immunohistochemistry: Tricks Of The Trade. Rodney T
Miller,M.D.
Immunohistochemistry, ProPath Laboratory, Inc. 8267 Elmbrook drive, Suite
100,
Dallas, Tx 75247-4009, (w) 214-237-1631 e-mail: rtmiller@ont.com

"In 1994 Sherman et al described a method of cell transfer can faciltate
immunostaining of small cytologic specimens, when only one or two slides are
available. This involves removing the coverslips from the slides by soaking
in
xylene, then covering the slides with liquid coverglass medium and heating
them, so that the medium hardens. (The liquid coverglass medium, called "
Mount Quick can be obtained from Newcomer Supply, 2217B Parview Road,
Middleton, Wi 53562, 608-831-7888, 800-383-7799, fax 608-931-0866). The
slides
are then placed in warm water for an hour or so, and this allows the removal
of the liquid coverglass medium along with the attached cells. The
coverglass
medium is then cut into smaller pieces with a razor blade, and each of these
pieces is transferred to a different adhesive slide, along with appropriate
control material on the same slide if possible. The slide are then dried in
an
oven, and the liquid coverglass material in then removed by soaking the
slides
in xylene. The slides are then rehydrated, the immunostains are performed on
the slides. We have used this technique countless times in our lab, and it
works extremely well for us. (In one case we were able to perform 20
immunostains on material from a single previously stained FNA cytologic
smear!) In 1993 Mehta an Battifora described a similar technique called the
"peel and stick" method for use with paraffin sections, employing a liquid
coverslip medium called "Harleco Krystalon"(EM Diagnostic Systems,
Inc.,Gibbstown, NJ). The "peel and stick" technique has minor differences
but
is similar in principal to the "cell transfer" technique described above. We
have also used the "cell transfer" technique extensively on previously
stained
H&E tissue sections, so indeed it works just as well for histologic sections
as cytologic smears. It is particularly useful on small needle biopsies of
prostate, where the tiny suspicious areas frequently disappear on deeper
sections, necessitating use of H&E levels for tissue transfer and subsequent
immunostaining."

Sherman ME, Jimenez-Joseph D, Gangi MD et al:Immunostaining of small
cytologic
specimens. Facilitation with cell transfer. Acta Cytologica 38(1):18-22,
1994.

Mehta P, Battifora H: How to do Multiple immunostains when only one tissue
slide is available. The "peel and stick" method. Applied
Immunohistochemistry
1(4): 297-8, 1993.

Dan
Windsor Regional Hospital


P. Emry wrote:

> Hi friends,
>
> I have lost a block.  I have sections from it on slides that didn't hold
> up during
> the brdu process, tissues came off the slides. The slides were ones that I
> had
> coated myself with silane.  Now I have the super plus slides I bought and
> wanted to cut some sections using them to see if we could get the tissues
> to stay on them..alas, lost the block.
>
> Is there any way to transfere the tissues off of the first slides on to
> the second slides or some treatment of the first slides with the tissues
> on them that would help prevent the tissues from falling off?
>
> Thanks,
> Trisha
> UWA







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