RE: not a strange request on mouse aorta cultures
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From: | "Weems, Joyce" <JWEEMS@sjha.org> |
To: | "'Smith, Allen'" <asmith@mail.barry.edu>, "'Gayle Callis'" <uvsgc@msu.oscs.montana.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
I don't have this publication - would you mind faxing a copy to
404-851-7831? Please and many thanks!!!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
-----Original Message-----
From: Smith, Allen [SMTP:asmith@mail.barry.edu]
Sent: Tuesday, April 25, 2000 4:51 PM
To: 'Gayle Callis'
Cc: 'histonet@pathology.swmed.edu'
Subject: RE: not a strange request on mouse aorta cultures
Carnoy's fluid without chloroform is Clarke's fluid. See Kiernan's
HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., p. 27.
Allen A. Smith
Barry University
Miami Shores, FL
-----Original Message-----
From: Gayle Callis [mailto:uvsgc@msu.oscs.montana.edu]
Sent: Monday, April 24, 2000 7:17 PM
To: histonet@pathology.swmed.edu
Subject: not a strange request on mouse aorta cultures
Have done this, they wanted to see the vascular (actually
angiogenisis)
development off the aortic ring, and the fibrin and collagen gel
really did
not interfere with the morphology of the ring or the vessels that
grew off
of it.
We fixed our rings, intact on the culture plate, basically fixing
them in
place in the gel contained in round culture dishes, using METHCARN,
methyl
alcohol/Carnoys, or the fixative of choice. We did not put
chloroform in
the fixative, too toxic, and not needed, no fat removal involved.
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