RE: not a strange request on mouse aorta cultures

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From:"Weems, Joyce" <JWEEMS@sjha.org>
To:"'Smith, Allen'" <asmith@mail.barry.edu>, "'Gayle Callis'" <uvsgc@msu.oscs.montana.edu>
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I don't have this publication - would you mind faxing a copy to
404-851-7831? Please and many thanks!!!

Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Smith, Allen [SMTP:asmith@mail.barry.edu]
	Sent:	Tuesday, April 25, 2000 4:51 PM
	To:	'Gayle Callis'
	Cc:	'histonet@pathology.swmed.edu'
	Subject:	RE: not a strange request on mouse aorta cultures

	Carnoy's fluid without chloroform is Clarke's fluid.  See Kiernan's
	HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., p. 27.
						Allen A. Smith
						Barry University

						Miami Shores, FL
	-----Original Message-----
	From: Gayle Callis [mailto:uvsgc@msu.oscs.montana.edu]
	Sent: Monday, April 24, 2000 7:17 PM
	To: histonet@pathology.swmed.edu
	Subject: not a strange request on mouse aorta cultures


	Have done this, they wanted to see the vascular (actually
angiogenisis)
	development off the aortic ring, and the fibrin and collagen gel
really did
	not interfere with the morphology of the ring or the vessels that
grew off
	of it.

	We fixed our rings, intact on the culture plate, basically fixing
them in
	place in the gel contained in round culture dishes, using METHCARN,
methyl
	alcohol/Carnoys, or the fixative of choice.  We did not put
chloroform in
	the fixative, too toxic, and not needed, no fat removal involved.   
	



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