Carnoy's fluid without chloroform is Clarke's fluid.  See Kiernan's
HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., p. 27.
					Allen A. Smith
					Barry University		
					Miami Shores, FL
-----Original Message-----
From: Gayle Callis [mailto:uvsgc@msu.oscs.montana.edu]
Sent: Monday, April 24, 2000 7:17 PM
To: histonet@pathology.swmed.edu
Subject: not a strange request on mouse aorta cultures


Have done this, they wanted to see the vascular (actually angiogenisis)
development off the aortic ring, and the fibrin and collagen gel really did
not interfere with the morphology of the ring or the vessels that grew off
of it.

We fixed our rings, intact on the culture plate, basically fixing them in
place in the gel contained in round culture dishes, using METHCARN, methyl
alcohol/Carnoys, or the fixative of choice.  We did not put chloroform in
the fixative, too toxic, and not needed, no fat removal involved.