RE: frozen embryos

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From:Garry Ashton <GAshton@picr.man.ac.uk>
To:"'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
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Histology Department
Paterson Institute
Wilmslow Road
Manchester
M20 9BX
UK

-----Original Message-----
From:	Garry Ashton
Sent:	27 April 2000 13:46
To:	'histonet@pathology.swmed.ed'
Subject:	frozen embryos

Dear all,
I am having a couple of problems with the freezing down of some mouse 
embryos.
Firstly can anybody suggest the best way to freeze down unfixed (about 
day 14) embryos without them cracking.
Secondly what's the best way to freeze down fixed embryos. The problem 
with these is that if I cryoprotect them with 30% sucrose, this 
concentrates in the body cavities and then prevents me from sectioning 
them.
Thanks for any help in advance
Garry

Histology Department
Paterson Institute for Cancer Research
Wilmslow Road
Manchester
M20 9BX
UK




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