I presume that the dye discussed is Cresyl Violet acetate. The perchlorate 
version has a chemical group removed to make it suitable as a laser dye. Our CV 
acetate has a 69% dye contents and the certificate by the Biological Staining 
Commission says "Satisfactory for metachromatic, nuclear and nissl staining. "

It is not usual practice to quote pricing on a listserver, but I am astonished. 
Lorraine quoted fantastic prices for an apparently very similar and probably 
identical product as ours. We list that item for A$83 per 10g - which is about 
US$48 and that is somewhat removed from US$222. Often price is related to 
quality, but "it ain't necessarily so . . "  What is more, if the Commission 
finds the material satisfactory for certain procedures, it would appear 
wasteful to for instance, substitute the rather more expensive perchlorate 
laser dye.
Cheers
Jim Darley
ProSciTech                 Microscopy PLUS
PO Box 111, Thuringowa  QLD  4817  Australia
Ph +61 7 4774 0370  Fax:+61 7 4789 2313  service@proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
                      www.proscitech.com

On Saturday, April 29, 2000 3:50 AM, L. Gibbs [SMTP:lgibbs@u.washington.edu] 
wrote:
> I only use Cresyl Fast Violet produced by Chroma-Gesellschaft,
> Schmid and distributed by Cell Point Scientific. Cell Points
> phone= (800) 999-9734. The catalog # is 1A396, specify 10 or 25
> grams, $222, $ 441.55.
> Sorry, I am not familiar with using Cresyl Violet for feulgen
> staining. I use the above Cresyl for Nissl staining.
>
> Good luck-
>
> Lorraine Gibbs
> University of Washington
>
> On Fri, 28 Apr 2000, Yamamoto, Karen wrote:
>
> > does anyone have any experience with using cresyl violet for feulgen
> > staining?
> > does anyone have any recommended vendors for high purity cresyl violet?
> > does anyone have any experience with differences in 70%-ish purity vs 100%
> > purity dye lots of cresyl violet with respect to feulgen staining for dna
> > quantitation?
> >
> >
> >
> > Karen K. Yamamoto, Ph.D.
> > Director Assay Development
> > ChromaVision Medical Systems, Inc.
> > 33171 Paseo Cerveza
> > San Juan Capistrano, CA  92675
> >
> > 949.443.3355 ext. 215
> > 949.443.3366 fax
> >
> >
> >
>