RE: Ventana ES & genII users

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From:Jeff & Wanda Gray <>
To:"Drew Sally A." <>, 'Histonet' <>
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At our hospital we run the immunos down a modified counterstain program on
our Sakuras after the Nexes is finished with them.  Our docs do not like the
Ventana hemo at all, so we lightened up our routine hematoxylin counterstain
by a minute or two, and just run 'em down the stainer.  We use Richard Allen
hematoxylin, but I am at home and don't remember off the top of my head
which one.  If you would like to know, contact me and I will find out.
Wanda Shotsberger

-----Original Message-----
From: Drew Sally A. []
Sent: Tuesday, April 18, 2000 11:41 AM
To: 'Histonet'
Subject: Ventana ES & genII users

We were wondering what type of counterstain do you use on your
instrument...Do you use Ventana's hematoxylin or a homebrew?  We have been
using a homebrew-diluted commercial Harris hematoxylin.  Our diluent is
composed of 128 mL Type 1 water, 60 mL 20% polyethylene glycol, and
12 mL glacial acetic acid.  Since we feel the diluted stain is fading too
much in the dispensers, we're kind of looking for a replacement protocol.
We would welcome any and all suggestions!
Thank you!

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI

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