We use the Ventana hematoxylin.  We have no complaints with the stain but
with the dispensers.  They run out earlier than expected.

> ----------
> From: 	Drew Sally A.[SMTP:sa.drew@hosp.wisc.edu]
> Sent: 	Tuesday, April 18, 2000 11:41 AM
> To: 	'Histonet'
> Subject: 	Ventana ES & genII users
> 
> We were wondering what type of counterstain do you use on your
> instrument...Do you use Ventana's hematoxylin or a homebrew?  We have been
> using a homebrew-diluted commercial Harris hematoxylin.  Our diluent is
> composed of 128 mL Type 1 water, 60 mL 20% polyethylene glycol, and 
> 12 mL glacial acetic acid.  Since we feel the diluted stain is fading too
> much in the dispensers, we're kind of looking for a replacement protocol.
> We would welcome any and all suggestions!
> Thank you!
> 
> Sally Ann Drew-MT(ASCP)
> Univ. of Wis. Hospital and Clinics
> IHC/ISH Clinical/Research Lab
> Madison, WI 
> (608)265-6596
> (608)263-1568(fax)
> 
>