Dear Andrea,
I have used Histogel for my cell "buttons" and have had no problem. Give
it a try>I used the same procedure as you, spinning the starting at 70%.
I do not have to stain for lipids though and my cells are fixed in 10%
NBF.
Betsy Molinari
Texas Heart Institute

> ----------
> From: 	Andrea Grantham[SMTP:algranth@U.Arizona.EDU]
> Sent: 	Wednesday, April 26, 2000 5:46 PM
> To: 	ValleyGal@aol.com
> Subject: 	Fwd: Lipid stain question/embedding cells answer
> 
> 
> >Date: Wed, 26 Apr 2000 15:20:49 -0700
> >To: Histonet
> >From: Andrea Grantham <algranth@u.arizona.edu>
> >Subject: Lipid stain question/embedding cells answer
> >
> >I've been asked to do a stain for lipids on cells grown on a glass
> slide. 
> >Has anybody out there done this or does anybody have a suggestion of
> what 
> >stain to use?
> >I was thinking Sudan Black. Will this work if I fix the cells on this
> 
> >slide in 10% formalin and then proceed on with the stain procedure as
> it 
> >is in the green AFIP manual? (Sudan made up with propylene glycol).
> >
> >Embedding cells - I have done this several times but I process the
> cells 
> >on my processor. I get the cell lines and fix them in either alcohol
> or 
> >formalin and then spin it down to form the button. I have wrapped the
> 
> >button in lens paper and put it in a cassette or I have used a
> histoscreen 
> >cassette.
> >I just got my sample of histogel and was thinking of using this the
> next 
> >time I have to do one of these.
> >Processing is as usual, starting in 70% ETOH.
> >Usually the cells stick together in the button and after processing I
> 
> >embed as usual.
> >
> >Thanks!
> 
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  histo@u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
>