RE: Help Needed

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From:"Colbert, Laurie" <>
To:"'Hagerty, Marjorie A.'" <>, "'Histonet'" <>
Content-Type:text/plain; charset="iso-8859-1"


In our quest to solve our processing problem I was told that alcoholic
fixation would give a more intense hematoxylin stain - that the chromatin
would look denser and darker.  Are you starting your processing run with
formalin or alcohol?  Just a thought - good luck!

Laurie Colbert
Saint Joseph Medical Center
Burbank, CA

-----Original Message-----
From: Hagerty, Marjorie A. []
Sent: Thursday, April 27, 2000 1:06 PM
To: 'Histonet'
Subject: Help Needed


I have a problem which I have never encountered before. The tissue coming
off our rush machine, mostly small biopsies and bone marrow blocks, is not
right. The nuclei are muddy and the epithelium a little blown out. They are
extremely basophilic picking up the hematoxylin very intensely. 

Any one have any experience with this? All temperatures check out. The
problem seems to be the during the processing.

We plan to change every solution on the machine, but I sure would like to
know what causes this. Any ideas/help would be greatly appreciated.

Perplexed in Palm Springs

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