LR White staining
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From: | "Jeff Crews"<jcrews@organo.com> |
To: | <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Howdy ho!
We've been using JB4+ for embedding some collagenous vascular
constructs, and have been pretty pleased with the results. However,
we're testing the possibility of using LR White for the same purpose
since it doesn't have to be mixed, there's no benzoyl peroxide, etc.
I like how the LRW sections, but I am having a hard time staining it.
For the JB4, we just use a quick stain of tol blue w/ borax, stain
on the hot plate for about a minute, rinse, differentiate with water,
and that's it. It works great for the JB4, but the results are highly
variable for LR White.Sometimes it stains well, sometimes patchy, and
sometimes I swear we get negative staining: the resin stains and the
collagen does not.
What are you all using for a quick stain of LR White sections (1.5
micron)? From my experience so far we'd switch, but the staining
problem is stopping me. I'd love to hear what you think.
Jeffrey Crews, HTL (ASCP)
Organogenesis, Inc.
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