LR White staining

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From:"Jeff Crews"<>
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     Howdy ho!
        We've been using JB4+ for embedding some collagenous vascular 
     constructs, and have been pretty pleased with the results. However, 
     we're testing the possibility of using LR White for the same purpose 
     since it doesn't have to be mixed, there's no benzoyl peroxide, etc.
     I like how the LRW sections, but I am having a hard time staining it. 
        For the JB4, we just use a quick stain of tol blue w/ borax, stain 
     on the hot plate for about a minute, rinse, differentiate with water, 
     and that's it. It works great for the JB4, but the results are highly 
     variable for LR White.Sometimes it stains well, sometimes patchy, and 
     sometimes I swear we get negative staining: the resin stains and the 
     collagen does not. 
        What are you all using for a quick stain of LR White sections (1.5 
     micron)? From my experience so far we'd switch, but the staining 
     problem is stopping me. I'd love to hear what you think.
     Jeffrey Crews, HTL (ASCP)
     Organogenesis, Inc.  

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