> -----Original Message-----
> From:	Merriweather, Annie (OD/ORS) 
> Sent:	Monday, April 17, 2000 8:21 AM
> To:	'histonet@pathology.swmed.edu'
> Subject:	Green Fluorescent Protein
> 
> 
> Dear Histonet friends,
> I'm looking for help with the best fixation/processing methodologies for
> histology of tissues expressing fluorescence from Enhanced Green
> Fluorescent
> Protein (a Clontech product).  
> 
> An investigator doing gene-transfer research has transplanted autologous
> bone marrow that had been transfected with the GFP gene into a rhesus
> monkey.  He wants to be able to take biopsies from the monkey, fix the
> tissue, embed and section it for fluorescence microscopy.  Alcohols
> apparently kill the signal -
> which makes embedding problematic.  
> 
> Has anyone successfully done fluorescence microscopy on fixed tissue
> sections which demonstrate which cells are expressing the GFP? 
> Thanks in advance your help,
> Annie 
> am98a@nih.gov
> The National Institutes of Health
> 
> 
> 
> 
> 
>