Embedding cells

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From:Emma Carter <E.Carter@OxfordBioMedica.co.uk>
To:"'histonet'" <histonet@pathology.swmed.edu>

I am sure i read a message about this recently, but i cant recall, and i
dont have anything in my mail folder...

someone has asked me about paraffin embedding some cells. I came up with a
protocol off the top of my head, roughly:

Put cells in eppendorf tube, spin down and remove culture buffer.
Resuspend with formalin and leave for 10 mins. Spin down and remove
Resuspend/spin down through serial alcohols, then xylene.
after final resuspension with xylene, remove and cover with warm wax.
Allow wax pellet to set. Pop out of tube and embed in cassette.

How does this appear to other people? Should i go through the dehydration

Any help would be very appreciated...... (oh yeah, this is all to be done by
my own fair hand.......automation!? Pah!)

Thanks in advance,

emma carter

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