Clarkes vs Carnoys without chloroform

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From:Gayle Callis <>
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Clarkes fixative is SIMILAR to (NOT the same!) as Carnoys without the
addition of chloroform, but if you do a calculation to check on per cent of
acetic acid in the alcohol, you will come up with:

v/v, results in per cent

Clarkes  results in 25% acetic acid 

25 ml acetic acid
75 ml 100% ethanol

Carnoys without the 30 ml of chloroform results in 16.67% acetic acid, 

10 mls acetic acid
60 mls 100% ethanol

There is another fixative that is 5% acetic acid in 95% ethanol, called
Meiers fixative, and actually refers to the author of the publication, and
is used to produce wonderful immunofluorescent IgG, IgA, and IgM staining
on paraffin sections fixed with this fixative.

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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