Citrate buffers and measuring pH

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From:"Nicola Falconer" <>
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dear Louise and all

Another late reply! We make citrate buffer in 10 litre amounts and it keeps
OK at room temp for a couple of weeks. We use the working stuff for 1 week
topping up as required, so it probably gets changed evry other day.

For measuring ph of buffers, maybe I'm paranoid, but we use a real pH meter,
no diddly stick ones, calbrated weekly, and measure the pH when making the
buffers up. We check our TBS for diluting antibodies EVERY day with above

For those who use other methods, if it ain't broke don't fix it, BUT if you
have problems with consistancy, guess what I recommend you check first.

John Auld
Royal Free Hospital

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