I'm going to be running an immuno stain for COX-2 on rat and mouse bone 
fractures.  I know there are a lot of you out there who run this stain, and 
was looking for any suggestions that you may have.  We're using a polyclonal 
rabbit primary antibody to murine COX-2 (Cayman Chemical Company), a 
secondary anti-rabbit conjugated to HRP, and DAB as our chromagen.  I was 
planning on using our normal blocking procedure:  3% hydrogen peroxide for 
endogenous peroxidase, and 3% powdered milk in 1% BSA to block non-specific 
sites.  Is antigen retrieval recommended for COX-2?  If so, what type?

Any help is greatly appreciated.

Thanks,

Jay


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Jay D. Turner
Dept. of Orthopaedics
University of Medicine and Dentistry of New Jersey
185 South Orange Ave.
Newark, NJ 07103
Phone: 973-972-4408
Fax: 973-972-5294
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