immuno TEM?

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From:Karen P Maruska <>
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Can anyone point me in the right direction for a protocol to do
immunocytochemistry followed by transmission electron microscopy (TEM)?

we need to provide evidence for synaptic contact between cells and
demonstrate that the synaptic vesicles contain a specific neuropeptide 
(this will be done in the fish brain and we have a primary antibody for
the peptide).

any suggestions would helpful!  thanks in advance!

Karen Maruska

Karen P. Maruska
Department of Biological Sciences
Florida Institute of Technology
150 W. University Blvd.
Melbourne, FL.  32901
phone: 321-674-7301
fax:   321-674-8255

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