immuno TEM?
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From: | Karen P Maruska <maruska@winnie.fit.edu> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Can anyone point me in the right direction for a protocol to do
immunocytochemistry followed by transmission electron microscopy (TEM)?
we need to provide evidence for synaptic contact between cells and
demonstrate that the synaptic vesicles contain a specific neuropeptide
(this will be done in the fish brain and we have a primary antibody for
the peptide).
any suggestions would helpful! thanks in advance!
Karen Maruska
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Karen P. Maruska
Department of Biological Sciences
Florida Institute of Technology
150 W. University Blvd.
Melbourne, FL. 32901
email: maruska@fit.edu
phone: 321-674-7301
fax: 321-674-8255
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