immuno TEM?

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From:Karen P Maruska <maruska@winnie.fit.edu>
To:histonet@pathology.swmed.edu
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Can anyone point me in the right direction for a protocol to do
immunocytochemistry followed by transmission electron microscopy (TEM)?

we need to provide evidence for synaptic contact between cells and
demonstrate that the synaptic vesicles contain a specific neuropeptide 
(this will be done in the fish brain and we have a primary antibody for
the peptide).

any suggestions would helpful!  thanks in advance!


Karen Maruska

*******************************
Karen P. Maruska
Department of Biological Sciences
Florida Institute of Technology
150 W. University Blvd.
Melbourne, FL.  32901
email: maruska@fit.edu
phone: 321-674-7301
fax:   321-674-8255
*******************************





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