<< Previous Message | Next Message >>
From:Gayle Callis <>
Content-Type:text/plain; charset="us-ascii"

I know that some people have used household gelatin found in kitchens

The size of the molecule was always a question, or bloom, as Sigma and
other chemcical catalogs, state it.  The household gelatin always seemed
coarse, and when a few granules were put in waterbath, did not always
dissolve.  Gelatin for microbiology purposes is also used, not sure of the

Decided to become more scientific, and started to use the following.

For soft tissues, in waterbath or normal subbing Type A,75-100 bloom,
smaller molecule, from bovine skin is used

For bone, decalcified sections, 225 bloom, Type B, from bovine skin or 300
bloom, Type A from porcine skin is used.  These both will give excess
background staining, unless cross linked a bit with NBF on subbed slides.
These also work in Haupt's gelatin for bone, the really difficult sections.

Oh dear, another lecture

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

<< Previous Message | Next Message >>