anti-calretinin and pale brown DAB reaction

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From:Tibor Ric <>
To:HistoNet Server <>
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Hi everybody!

I'm using anti- calretinin from Swant (Code No 7699/4, polyclonal) in my
project. The problem is that I'm getting too pale DAB reaction. I did
everything like before for HBME-1 and CK 5/6 and they were not that pale
brown. I'm sure that the DAB solution is okay. The material I'm using is
from 1991 to 1999.

One problem I consider here would be the fixation time which I don't know
for those older blocks.
Please, come with suggestions and good ideas for a beginner...

Today we tried a new antibody from Novocastra Laboratories (clone 5A5,
monoclonal) and I'm still having that pale brown color...

The positive controls were pale too.

Thanks in advance!

tibor ric
a Swedish student from the University of Karlstad, Sweden

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