anti-calretinin and pale brown DAB reaction
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From: | Tibor Ric <tibor@home.se> |
To: | HistoNet Server <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
Hi everybody!
I'm using anti- calretinin from Swant (Code No 7699/4, polyclonal) in my
project. The problem is that I'm getting too pale DAB reaction. I did
everything like before for HBME-1 and CK 5/6 and they were not that pale
brown. I'm sure that the DAB solution is okay. The material I'm using is
from 1991 to 1999.
One problem I consider here would be the fixation time which I don't know
for those older blocks.
Please, come with suggestions and good ideas for a beginner...
Today we tried a new antibody from Novocastra Laboratories (clone 5A5,
monoclonal) and I'm still having that pale brown color...
The positive controls were pale too.
Thanks in advance!
tibor ric
a Swedish student from the University of Karlstad, Sweden
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