Troubleshooting stains (2)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Histonet <Histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
14th April 2000
About a year ago I posted a message to HistoNet about a
book published in 1998 that looked as if it should be useful
to anyone doing staining. I would now like to give a more
informed opinion, having had the time to read pretty-well
everything in the book.
First of all, what is it? The title is:
"Troubleshooting Histology Stains," and it's by Richard W. Horobin
and John D. Bancroft. New York and Edinburgh: Churchill Livingstone.
pp. xvi + 266, with 36 colour plates. ISBN 0 443 05312 X. Hardback.
Needless to say, both authors are well known and have written other
books in the field.
A short introduction is followed by the coloured photos, which
illustrate tissues correctly stained by many methods. Collection of
these plates into a concentrated block of pages must have been the
publisher's idea, to reduce printing costs. It would make for easier
reading if each illustration accompanied the explanation of its
technique. There are 36 plates, each illustrating a different method.
It's unfortunate that 22 of the techniques discussed in the text
are not illustrated; perhaps this would have made the price
prohibitive.
The main part of the book contains accounts of 58 different
staining methods, in alphabetical order. Each account begins with
background information about how the method works. This is
followed by hints on avoiding problems (What types of specimen
processing are suitable? Is the procedure easy to carry out?)
and on dealing with problems that may arise (e.g. Staining
solution looks peculiar; Tissue stains unexpectedly weakly;
Unexpected structures stain). Each type of difficulty is
explained, with instructions for putting it right or avoiding
it next time. There are references in the text, which can be
followed up by referring to the 10-page bibliography.
Anyone intending to buy this book should realize that it does
not contain instructions for doing the staining methods. There
is a list (pp 257-261) of places to find practical instructions
for all the methods considered. (In almost every case this is
a page reference to Bancroft & Stevens, 1996.)
It seems to me that Horobin & Bancroft address their remarks to
those of us who have tried and failed when attempting to carry
procedures by following published instructions. This surely is
the experience of all Histonetters. Our merry listserver would
not exist if every published staining method provided a perfect
result, every time. H & B's book is a reference that anticipates
and provides answers for many questions of the kind that come
up on HistoNet. The book is quite expensive for its size, but
not unduly so for something in a specialized field. Books last
much longer than most laboratory items, and you could buy two
copies of this one for the price of a fairly ordinary pH meter.
It contains much information that cannot be found in print
anywhere else.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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