Re: trypsin digestion

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From:Dana Settembre <settembr@UMDNJ.EDU>
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On Wed, 5 Apr 2000 wrote:

> Hi everybody!
> I need various suggestions to do the trypsin digestion of my slides. I use PBS 7,4 and I'm going to digest my Ber-EP4 slides.
> Remember I'm a beginner, so sorry if my questions are stupid and not well asked.
> Thanks in advance!
> regards
> tibor
> _________________________________________________________
> En adress f#246#r livet registrerar du p#229#
Hi Tibor,
When I digest with trypsin I do it for about 15 minutes at 37 degrees
celcius.    I use Ber-EP4 too but I use prot. K for 5 minutes room
temperature. It is soo easy and comes ready to use from Dako.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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