Re: sequenza coverplate users

<< Previous Message | Next Message >>
From:Dana Settembre <settembr@UMDNJ.EDU>
To:"Harvey, Jennifer" <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Fri, 31 Mar 2000, Harvey, Jennifer wrote:

> I bought 4 sequenza rack and so far are not really happy with them. I am
> having a problem with faint staining. Someone suggested using triton X in by
> PBS and now I am getting background all over my slides. Do I need to do the
> rinses more that one time? When you converted over to using the coverplates
> did you have to retiter all of you antibodies? Any ideas would be helpful.
> Jennifer Harvey
> University of Rochester
Hello Jennifer
I occassionally do immunohistochemistry by hand and I use the Shandson
racks and coverplates.  You should rinse more than once. I rinse 2 times
at 5 minutes each.  I didn't re-titer either.  Good luck.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

<< Previous Message | Next Message >>