Re: sequenza coverplate users
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From: | Dana Settembre <settembr@UMDNJ.EDU> |
To: | "Harvey, Jennifer" <Jennifer_Harvey@URMC.Rochester.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Fri, 31 Mar 2000, Harvey, Jennifer wrote:
> I bought 4 sequenza rack and so far are not really happy with them. I am
> having a problem with faint staining. Someone suggested using triton X in by
> PBS and now I am getting background all over my slides. Do I need to do the
> rinses more that one time? When you converted over to using the coverplates
> did you have to retiter all of you antibodies? Any ideas would be helpful.
>
>
>
> Jennifer Harvey
> University of Rochester
> jennifer-harvey@urmc.rochester.edu
>
Hello Jennifer
I occassionally do immunohistochemistry by hand and I use the Shandson
racks and coverplates. You should rinse more than once. I rinse 2 times
at 5 minutes each. I didn't re-titer either. Good luck.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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