Re: immuno TEM?

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Karen wrote:
>Can anyone point me in the right direction for a protocol to do
>immunocytochemistry followed by transmission electron microscopy (TEM)?
>we need to provide evidence for synaptic contact between cells and
>demonstrate that the synaptic vesicles contain a specific neuropeptide
>(this will be done in the fish brain and we have a primary antibody for
>the peptide).
Dear Karen,
Pre-embedding immunolabeling using ultra-small gold conjugates and silver
enhancement is an option. Please visit our web-site for additional info.
Hong Yi from Emory University used this technique for the detection of a
variety of antigens in rat brain vibratome sections.

Peter van de Plas
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955

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