Re: gelatin

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From:Charles Eisener <>
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One problem with gelatin powder is controlling the actual amount dispensed.
For those of us with large fingers, a "pinch" is more substantial than may
be typical. The actual source of gelatin is not critical - we use lab grade
(Fisher G8) Type A.

A 1% solution is made in distilled water, and 1.0 ml dispensed into each
waterbath via disposable pipette. The stock is heated in the microwave
before dispensing. Larger amounts have resulted in background staining with
some methods.

Charles Eisener
Laboratory Alliance of Central New York
Syracuse, NY
Charles Eisener
----- Original Message -----
From: "Gayle Callis" <>
To: <>
Sent: Thursday, April 06, 2000 8:52 PM
Subject: gelatin

> I know that some people have used household gelatin found in kitchens
> The size of the molecule was always a question, or bloom, as Sigma and
> other chemcical catalogs, state it.  The household gelatin always seemed
> coarse, and when a few granules were put in waterbath, did not always
> dissolve.  Gelatin for microbiology purposes is also used, not sure of the
> bloom.
> Decided to become more scientific, and started to use the following.
> For soft tissues, in waterbath or normal subbing Type A,75-100 bloom,
> smaller molecule, from bovine skin is used
> For bone, decalcified sections, 225 bloom, Type B, from bovine skin or 300
> bloom, Type A from porcine skin is used.  These both will give excess
> background staining, unless cross linked a bit with NBF on subbed slides.
> These also work in Haupt's gelatin for bone, the really difficult
> Oh dear, another lecture
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
> 406 994-4705
> 406 994-4303

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