Re: PH of IHC buffers

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From:Becky Scholes <>,,, settembr@UMDNJ.EDU
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Lori Steele from Biogenex just gave a lecture at our State meeting.  She 
said that a pH meter is not necessary to check the buffer solution for 
immunohistochemical staining - just use a pH tape with 1.0 intervals.  
However, it still needs to be done each day you do IHC staining.  I know in 
our busy lab, it sure is quicker than having to use the meter.

Becky Scholes
Iowa Pathology Associates
Des Moines, Iowa

>From: Joyce Kotzuk <>
>To:,, settembr@UMDNJ.EDU
>Subject: Re: PH of IHC buffers
>Date: Tue, 04 Apr 2000 15:10:32 -0600
>I calibrate my pH meter once a day.  Is this overkill? I do it because 
>that's what was done in my old lab, not because I know for sure that's what 
>needs to be done. How often do you all out there calibrate? Thanks,
>Joyce Kotzuk, UNM pathology dept.
> >>> Dana Settembre <settembr@UMDNJ.EDU> 03/30/00 11:30AM >>>
>On Wed, 29 Mar 2000, anita dudley wrote:
> > Would anyone out there let me know what they use to check the ph of 
> > IHC buffer.  It is a question on the CAP checklist, we have dip sticks 
>but I
> > just wonder if other people are using a ph meter and what kind and is it
> > checked every day?  thanks so much for the info
> >
> > Anita Dudley
> > Providence Hosp
> > Mobile Ala
> > ______________________________________________________
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> >
>Hi Anita,
>I use a pH meter by Corning.  I make buffer about once or twice a week.  I
>pH each time I make it.  I calibrate my pH meter once a month.
>Dana Settembre
>Immunohistochemistry Lab
>Pathology Department
>University Hospital
>Newark,  New Jersey

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