Re: PH of IHC buffers
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From: | Becky Scholes <raws43@hotmail.com> |
To: | JKotzuk@salud.unm.edu, azdudley@hotmail.com, histonet@pathology.swmed.edu, settembr@UMDNJ.EDU |
Reply-To: | |
Content-Type: | text/plain; format=flowed |
Lori Steele from Biogenex just gave a lecture at our State meeting. She
said that a pH meter is not necessary to check the buffer solution for
immunohistochemical staining - just use a pH tape with 1.0 intervals.
However, it still needs to be done each day you do IHC staining. I know in
our busy lab, it sure is quicker than having to use the meter.
Becky Scholes
Iowa Pathology Associates
Des Moines, Iowa
>From: Joyce Kotzuk <JKotzuk@salud.unm.edu>
>To: azdudley@hotmail.com, histonet@pathology.swmed.edu, settembr@UMDNJ.EDU
>Subject: Re: PH of IHC buffers
>Date: Tue, 04 Apr 2000 15:10:32 -0600
>
>I calibrate my pH meter once a day. Is this overkill? I do it because
>that's what was done in my old lab, not because I know for sure that's what
>needs to be done. How often do you all out there calibrate? Thanks,
>Joyce Kotzuk, UNM pathology dept.
>
> >>> Dana Settembre <settembr@UMDNJ.EDU> 03/30/00 11:30AM >>>
>On Wed, 29 Mar 2000, anita dudley wrote:
>
> > Would anyone out there let me know what they use to check the ph of
>their
> > IHC buffer. It is a question on the CAP checklist, we have dip sticks
>but I
> > just wonder if other people are using a ph meter and what kind and is it
> > checked every day? thanks so much for the info
> >
> > Anita Dudley
> > Providence Hosp
> > Mobile Ala
> > ______________________________________________________
> > Get Your Private, Free Email at http://www.hotmail.com
> >
> >
>Hi Anita,
>I use a pH meter by Corning. I make buffer about once or twice a week. I
>pH each time I make it. I calibrate my pH meter once a month.
>
>Dana Settembre
>Immunohistochemistry Lab
>Pathology Department
>University Hospital
>Newark, New Jersey
>USA
>
>
>
>
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