Re: Negative Controls for Immunohistochemistry

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From:Debbie Pepperal <dpeppera@mail.newcastle.edu.au>
To:ToniT41@aol.com
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hi Toni

Rather late than never. We always run negative control slides by omitting
the primary antibody, and depending on what antigen retrieval system is
used at the moment, ( we run one for each different protocol used) as well
as  whether there are polyclonal and monoclonal antibodies to be run , as
there secondary antibodies would be different and that checks all levels of
detection. 

Zenobia Haffajee
HAPS, Newcastle, NSW, Australia

Hope this adds up to all the other answers you may have received. At 04:03
PM 06/04/00 -0400, you wrote:
>I would like to know what everyone is using for negative controls?
>
>For example are you using a known positive control slide for mouse or rabbit 
>negative antibody?
>or known negative slides against the antibody itself?
>and is everyone using the patient control with mouse or rabbit negative 
>antibody?
>
>I guess I'm trying to find out how labs are handleing negative controls?
>
>Thanks
>Toni
>
>



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