Re: IHC double labeling, TUNEL/p21

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From:Debbie Pepperal <>
To:"Philopena, Jennifer" <>
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Hi Jen

Strangely so....I was looking for a  similar article. I am also doing the
TUNEL technique (Boehringer Mannheim) and intend to do double labelling
soon. However , I find that with the TUNEL technique, staining  is not
totally optimal, in that: although most of the apoptotic cells are staining
beautifully, there are a few cells that look apoptotic but are negative.
Can't quite explain it! Have you had any similar experiences?
The journal article I found was:
J of histochemistry and Cytochemistry, Vol 44. No 9; pages 959-968; 1996.
If you had a more recent publication, please let me know.
Have fun.

Best Regards

Zenobia Haffajee
HAPS, Newcastle, Australia

 At 02:42 PM 29/03/00 -0500, you wrote:
>Hi.  I'm looking for a procedure that would allow me to label with an
>anti-p21 antibody (we use the Pharmingen monoclonal) and also use the TUNEL
>assay on the same section.  I've seen it in literature, just can't find it
>presently.  Thanks a lot.  Jen

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