Re: Fluorescent immunohistochemistry
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From: | DDittus787@aol.com |
To: | Jennifer.Johnson@genzyme.com, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
I have done some IF work, yes leaving the antibody on too long will yield
high background and pick up dust particles , etc. Also some flourescent
antibodies do not need secondary or pre-treatments,third you should use a
water soluble chromagen for one antibody, then the flourescent antibody, most
flourescent antibodies are coverslipped with a water based mountant, not
great for permanent
slides. Dana
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