Joe,

Try using a species-specific secondary ( I guess I'm assuming you are using 
a poly-valent secondary). We were having terrible background problems and 
traced it to the secondary in the DAKO kit. A species-specific secondary did 
the trick.



Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043


----Original Message Follows----
From: "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
To: 'Histonet' <histonet@pathology.swmed.edu>
Subject: Dako Autostainer
Date: Mon, 03 Apr 2000 10:16:55 -0500

Ever say no to something then turn around and it happens to you.  My
pathologist discussed some immuno problems with me today.  Yep, I have a
Dako autostainer.  Yep, I'm starting to have problems.  Yep, I called Dako.
That will be the last time I say "No, not me"
	On another note.  I'm using Dako's monoclonal p53 antibody.  We're
using breast cancer and colon cancer as controls.  We are picking up
collagen non-specifically.  Even performing an Avidin-Biotin block isn't
helping.  We re-titered the antibody so far out that we were missing weakly
positive nuclei.  We backed off on the titer and now are picking up
collagen.  Any ideas?  As always, thanks for your help.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas









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