Re: CryoJane & Gentle Jane
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | "Instrumedics, Inc." <cfss@idt.net> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Tue, 4 Apr 2000, Instrumedics, Inc. wrote:
> All the "Janes" are confusing! Clarification!
>
> ... tissue and CryoGel or other embedding medium. The block is frozen in
> 8-10 seconds. The temperature of the heat extractor,-196deg. C, and the
> thermal exchange derived from its highly polished chrome finish, produces
> "amorphous" ice rather than "crystalline" ice. minimizing ice crystal
> artifact. The morphology of the tissue is preserved!
You aren't going to get amorphous (i.e. vitreous) solid water by
this method, especially if it takes 8 seconds to freeze the
middle of the block, though the ice crystals may be too small
to make visible holes in the tissue. For vitrification you need
liquid helium temperature. See Bald, WB (1983) Optimizing the
cooling block for the quick freeze method. J Microsc 131: 11-23;
also Chapters 2 & 3 in Pearse's "Histochemistry," 4th edn, Vol 1.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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