Re: Acetic acid !
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Gamal Badawy <gmb505@bcssrv3.bham.ac.uk>, Histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Thu, 6 Apr 2000, Gamal Badawy wrote:
> I'm looking for an answer to this question:
> What is the role of acetic acid in Bouin's fixative and is it
> essential to be included in the formula for histology?
The acetic acid coagulates DNA and provides a characteristic
sharply defined pattern of chromatin distribution, often
characteristic for different cell-types. It also causes
swelling of the tissue (especially collagen), which is
counteracted by the picric acid in the mixture. Picric acid
combines with and coagulates proteins generally. The
formaldehyde cross-links proteins, mostly after they have
been coagulated, so it won't do much if you fix in Bouin
for only a few hours. A days is better, BUT with long
immersion the two acid components attack nucleic acids,
with extraction of RNA and production of aldehyde groups
in DNA (which becomes Schiff-positive). Anionic dyes work
well after Bouin (for reasons that could be discussed but
are largely speculative). Nuclei, however stained, are much
more pleasing to look at after Bouin (or other acetic acid
containing fixatives) than after neutral formaldehyde.
> Gamal Badawy
> Faculty of Science
> School of Biosciences
> University of Birmingham
I'm a Brum graduate, but there wasn't a School of
Biosciences in my time. Have they lumped some
traditional departments together?
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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