Stain problem:
Possibly you are not washing enough and have excess chemicals in the tissue 
section which may affect the stain as time goes on.

CryoJane.
This tool only helps you freeze the tissue well. It does not help you cut it 
better. For that the same company (Instrumedics) makes a tape transfer 
system that will help you get good sections from difficult tissue.  It takes 
a bit of practice to learn to use but then is a great help when you just 
can't get anything.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964

----Original Message Follows----
From: Buttigieg Carmen at MOH <carmen.a.buttigieg@magnet.mt>
To: histonet@pathology.swmed.edu
Subject: 1) Stains    2) Cryojane System
Date: Mon, 03 Apr 2000 11:54:55 +0200

Especially to Europeans

I have a few difficulties whith our H & Es. The Haematoxylin starts to fade
within a couple of years. Got any answers, like some miracle formula. I've 
been
hearing a lot about Richard-Allan's stains but I have no idea where they 
could
be purchased from. If they are available from a European country, it is more
convenient for us.
Another problem is cutting frozen sections containing a lot of fat. Someone
spoke about the Cryojane System. Would it solve my porblem? At the moment 
all I
get from fatty material are holes in the embedding medium. Again, if the is 
a
supplier somewhere in Europe, it is easier for me.
Thanks for all your help


Carmen
A/PLMT HISTOPATHOLOGY
ST. LUKE'S HOSPITAL
MALTA

carmen.a.buttigieg@magnet.mt






FAX:  (404)639-3043



______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com