RE: thin slides=longer staining

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From:"Colbert, Laurie" <>
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This is what I was taught, also - that there is less material to pick up the
stain, so we must stain thinner sections longer.

Laurie Colbert

-----Original Message-----
From: []
Sent: Friday, April 14, 2000 1:30 AM
Subject: thin slides=longer staining

I was a little surprised that more people didn't pick up on this--thinner 
slides (ex: 3 microns) need to be stained longer....there just isn't the 
amount of cellular material there to absorb stain....also, years ago when
special stains were developed the ability to cut sections at 3 microns had 
not been achieved..therefore, the times used in the stains were all
for those 5 micron sections....

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