RE: thin slides=longer staining
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From: | "Colbert, Laurie" <LColbert@phsca.org> |
To: | "'Cgs001@aol.com'" <Cgs001@aol.com>, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
This is what I was taught, also - that there is less material to pick up the
stain, so we must stain thinner sections longer.
Laurie Colbert
-----Original Message-----
From: Cgs001@aol.com [mailto:Cgs001@aol.com]
Sent: Friday, April 14, 2000 1:30 AM
To: histonet@pathology.swmed.edu
Subject: thin slides=longer staining
I was a little surprised that more people didn't pick up on this--thinner
slides (ex: 3 microns) need to be stained longer....there just isn't the
amount of cellular material there to absorb stain....also, years ago when
the
special stains were developed the ability to cut sections at 3 microns had
not been achieved..therefore, the times used in the stains were all
developed
for those 5 micron sections....
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