Peter,

I always perfused my mouse brains with zambonis or 4% para, whilst in situ,
having flushed through with Heparinised Saline for a couple of minutes
first.

I can give you precise details if you need them..

hope this is helpful,

emma carter

> -----Original Message-----
> From:	Peter Poon [SMTP:peterp@cafe.berkeley.edu]
> Sent:	Wednesday, April 12, 2000 7:28 AM
> To:	histonet
> Subject:	perfusion in mouse brain
> 
> Does anyone perfuse fixature and or dehydrating agents into mice brains?
> If so, does anyone use any type of vasodilator? If so what? and in what
> amounts?  and does anyone know of any articles that compares these
> vasodilators?
> 
> 
> 
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