Tamara is correct, and at the risk of offending someone, I consider this
neither good advice nor good laboratory technic. 

Gayle Callis 


>Date: Wed, 05 Apr 2000 17:27:15 -0400 (EDT)
>From: Tamara Howard <howard@cshl.org>
>Subject: RE:pH paper vs. meter
>To: raws43@hotmail.com, Histology listserver
>    <histonet@pathology.swmed.edu>
>
>>Lori Steele from Biogenex just gave a lecture at our State meeting.  She
>>said that a pH meter is not necessary to check the buffer solution for
>>immunohistochemical staining - just use a pH tape with 1.0 intervals.
>>However, it still needs to be done each day you do IHC staining.  I know
>>in our busy lab, it sure is quicker than having to use the meter.
>
>>Becky Scholes
>>Iowa Pathology Associates
>>Des Moines, Iowa
>
>Sorry, but this is not a good idea. Try for yourself - round up some pH
>papers - different ranges and vendors - and try all on one buffer. My bet
>would be that you'll get readings all over the place. Quicker may be nice,
>but reproducibility is really worth the extra time - and it takes less
>than 5 mintues to properly calibrate a meter and check your buffer's pH.
>
>Tamara Howard
>CSHL
>
>
>
>