RE: Richard-Allan Stains
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From: | "Greer, Bonnie" <Bonnie.Greer@stjude.org> |
To: | 'Robert Schoonhoven' <rschoonh@sph.unc.edu>, Linda Jenkins <jlinda@ces.clemson.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
I have been using them for 15 years with no problems.
-----Original Message-----
From: Robert Schoonhoven [mailto:rschoonh@sph.unc.edu]
Sent: Wednesday, March 29, 2000 3:43 PM
To: Linda Jenkins
Cc: histonet@pathology.swmed.edu
Subject: Re: Richard-Allan Stains
Linda,
'Twas the late '70s and yes I completely agree with you! Those are
great reagents and I have also used them since they came out. Still do
when I need an H&E or have to show a grad student what a good H&E is
supposed to look like (according to me).
Linda Jenkins wrote:
>
> Laurie Colbert wondered, "Is Richard Allan a common denominator
> with everyone who is having problems?????"
> My thoughts on this - I have used Richard-Allan hematoxylin,
eosin,
> clarifier, bluing, and cytology stains practically since their arrival in
> the marketplace (late '70's - mid-eighties?). The ONLY consistent,
> reliable procedure I've NEVER had to "tweak" in the last 20 years has been
> my H &E stain - as it is beautiful! In fact, in 1990, when I was first
> introduced to the gentleman (Jerry Fredenburg) who was responsible for the
> development of these wonderful chemicals I immediately hugged him and
said,
> "Thank You, Thank You, Thank You!" In fact, all of our area labs have
used
> these stains with great results.
> Now, in all fairness, I must add that I use real Xylenes for
> clearing and graded ethanols for dehydration. Have not been seen
> consistent results with substitutes.
> And, as a final note, I remember thinking that the original
> question that started this query had a processing procedure that went
> straight from formalin to 100%alcohol. Isn't this a little odd? Doesn't
> everyone use graded alcohols?
> Oh, well...I just didn't want techs, who've never used them, to
get
> the wrong impression about Richard-Allan stains.
> Linda
>
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> 864.656.5553
> **********************************
--
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123
Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)
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