RE: Richard-Allan Stains

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From:"Greer, Bonnie" <>
To:'Robert Schoonhoven' <>, Linda Jenkins <>
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I have been using them for 15 years with no problems.

-----Original Message-----
From: Robert Schoonhoven []
Sent: Wednesday, March 29, 2000 3:43 PM
To: Linda Jenkins
Subject: Re: Richard-Allan Stains


'Twas the late '70s and yes I completely agree with you!  Those are
great reagents and I have also used them since they came out.  Still do
when I need an H&E or have to show a grad student what a good H&E is
supposed to look like (according to me).

Linda Jenkins wrote:
>         Laurie Colbert  wondered, "Is Richard Allan a common denominator
> with everyone who is having problems?????"
>         My thoughts on this - I have used Richard-Allan hematoxylin,
> clarifier, bluing, and cytology stains practically since their arrival in
> the marketplace (late '70's - mid-eighties?).  The ONLY consistent,
> reliable procedure I've NEVER had to "tweak" in the last 20 years has been
> my H &E stain - as it is beautiful!  In fact, in 1990, when I was first
> introduced to the gentleman (Jerry Fredenburg) who was responsible for the
> development of these wonderful chemicals I immediately hugged him and
> "Thank You, Thank You, Thank You!"  In fact, all of our area labs have
> these stains with great results.
>         Now, in all fairness, I must add that I use real Xylenes for
> clearing and graded ethanols for dehydration.  Have not been seen
> consistent results with substitutes.
>         And, as a final note, I remember thinking that the original
> question that started this query had a processing procedure that went
> straight from formalin to 100%alcohol.  Isn't this a little odd?  Doesn't
> everyone use graded alcohols?
>         Oh, well...I just didn't want techs, who've never used them, to
> the wrong impression about Richard-Allan stains.
>         Linda
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> 864.656.5553
> **********************************

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

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