Do you want to decal the bone?  I can help you with methods for decalcified
bone processed in paraffin.  If you want to do calcified bone, MMA is the
way to go and Neil Hand is the expert on IHC on MMA embedded tissue.
Patsy Ruegg

-----Original Message-----
From: Jane Chambers [mailto:jane.chambers@Agouron.COM]
Sent: Tuesday, April 04, 2000 10:48 AM
To: HistoNet Server; Jane Chambers
Subject: IHC on bone


Due to a change in jobs and duties, I have been away from histonet for
almost a year.  Well I have been ask to look into doing
immunohistochemistry (IHC) on the knee joint of mice.  When I did IHC
previously it was not on bone.

My request is this:

Could I get some references on IHC on bone:  kits used; techniques;
books, etc.  I went to NSH in Salt Lake a couple of years ago and
remember a lot of workshops on IHC on bone so I am hoping someone could
pass some of this information on to me.

Thanks in advance,

Jane Chambers