> I'm trying to think of a method that needs longer times for thinner
>  sections but can't, unless the embedding medium is changed
> Are there some staining methods that take longer for thinner 
>sections in the same embedding medium? 


John,
How about a VG on a thin (2um) section? I seem to recall it took longer to 
stain the thin section than a thicker section in the same substrate. Which 
presumably is all to do with not much being there?
Or have I missed the point :)

Glyn