Dako Autostainer

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From:"Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
To:'Histonet' <histonet@pathology.swmed.edu>

Ever say no to something then turn around and it happens to you.  My
pathologist discussed some immuno problems with me today.  Yep, I have a
Dako autostainer.  Yep, I'm starting to have problems.  Yep, I called Dako.
That will be the last time I say "No, not me"
	On another note.  I'm using Dako's monoclonal p53 antibody.  We're
using breast cancer and colon cancer as controls.  We are picking up
collagen non-specifically.  Even performing an Avidin-Biotin block isn't
helping.  We re-titered the antibody so far out that we were missing weakly
positive nuclei.  We backed off on the titer and now are picking up
collagen.  Any ideas?  As always, thanks for your help.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

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